With the aim of understanding cellular interactions of hepatocytes in their physiological microenvironment and to generate an artificial 3D in vitro model of liver, a co-culture system using 3D extrusion bioprinting was developed. In this method, bio-ink based on alginate and methylcellulose was used.

reference:

Taymour R, Kilian D, Ahlfeld T, Gelinsky M, Lode A. 3D bioprinting of hepatocytes: core–shell structured co-cultures with fibroblasts for enhanced functionality. Scientific Reports. 2021 Mar 4;11(1):1-8.